A label-free LC/MS-based enzymatic activity assay for the detection of PDE5A inhibitors

A label-free LC/MS-based enzymatic activity assay for the detection of PDE5A inhibitors

Phosphodiesterase type 5 (PDE5), a cyclic nucleotide phosphodiesterase, controls the duration of the cyclic guanosine monophosphate (cGMP) signal by hydrolyzing cGMP to GMP. Inhibiting the activity of PDE5A has proven to be an effective strategy for treating pulmonary arterial hypertension and erect...

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Bibliographic Details
Main Authors: Yufeng Ma, Fengsen Zhang, Yijing Zhong, Yongchun Huang, Yixizhuoma, Qiangqiang Jia, Shoude Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-02-01
Series:Frontiers in Chemistry
Subjects:
PDE5A
LC/MS
cGMP
enzyme activity
inhibitor
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2023.1097027/full

Internet

https://www.frontiersin.org/articles/10.3389/fchem.2023.1097027/full

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