Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation
L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. Th...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2024-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/production-and-optimization-of-l-glutaminase-from-halophilic-fusarium-solani-melongenae-strain-cri-24-under-submerged-and-solid-state-fermentation/ |
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| author | M.S. Vineetha Nayef Abdulaziz Aldabaan Sunil S. More Mater H. Mahnashi Ibrahim Ahmed Shaikh Mohammad Shahzad Samdani Rashmi Swami Anirudh Yadav N. Rohith J. Bhavya Basheerahmed Abdulaziz Mannasaheb Mamdouh Saleh Alharbi Aejaz Abdullatif Khan Salah Eldeen Dafalla S.M. Shakeel Iqubal |
| author_facet | M.S. Vineetha Nayef Abdulaziz Aldabaan Sunil S. More Mater H. Mahnashi Ibrahim Ahmed Shaikh Mohammad Shahzad Samdani Rashmi Swami Anirudh Yadav N. Rohith J. Bhavya Basheerahmed Abdulaziz Mannasaheb Mamdouh Saleh Alharbi Aejaz Abdullatif Khan Salah Eldeen Dafalla S.M. Shakeel Iqubal |
| author_sort | M.S. Vineetha |
| collection | DOAJ |
| description | L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. This enzymatic process holds potential applications across diverse industries, notably in food and pharmaceuticals. The primary objective of the present research was to identify and isolate a fungal strain proficient in L-glutaminase production from soil found in maritime environments. The physical and nutritional conditions were optimized for maximum synthesis of L-glutaminase under solid state fermentation (SSF) and submerged fermentation conditions (SmF). The isolated organism was identified as Fusarium solani-melongenae strain CRI 24 by morphological and 18S rRNA analysis. The optimum carbon source under SmF and SSF was found to be starch (0.2% w/v). Wheat bran as solid substrate among others showed optimum enzyme activity. On the seventh day of incubation, at pH 8.0 and 0.7% L-glutamine concentration under SSF and SmF, the highest enzyme activity was detected. The greatest enzyme activity in SSF was seen at a moisture content of 10%. Fusarium solani-melongenae species produced the enzyme under optimal conditions and 4.20 and 4.73-fold increase (from 0.8 U/mL to 3.61 U/mL and from 0.781 U/mL to 3.69 U/mL) was achieved after optimization in submerged and in solid state fermentation, respectively. The selective isolation and optimization processes described in this work are a promising technique for the industrial production of L-glutaminase and can be applied in the pharmaceutical and food industries. |
| first_indexed | 2024-04-24T22:39:39Z |
| format | Article |
| id | doaj.art-cf9b45b2726f44489b5c151d43c9b5e3 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-24T22:39:39Z |
| publishDate | 2024-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-cf9b45b2726f44489b5c151d43c9b5e32024-03-19T07:20:29ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-03-01181593604https://doi.org/10.22207/JPAM.18.1.43Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State FermentationM.S. VineethaNayef Abdulaziz Aldabaanhttps://orcid.org/0009-0001-3349-9165Sunil S. Morehttps://orcid.org/0000-0001-5308-578XMater H. Mahnashihttps://orcid.org/0000-0002-3095-5604Ibrahim Ahmed Shaikhhttps://orcid.org/0000-0001-9812-2628Mohammad Shahzad Samdanihttps://orcid.org/0009-0001-4029-9794Rashmi Swamihttps://orcid.org/0009-0001-0721-6782Anirudh Yadavhttps://orcid.org/0009-0002-1618-8434N. Rohithhttps://orcid.org/0009-0008-5166-097XJ. Bhavyahttps://orcid.org/0000-0003-0586-2366Basheerahmed Abdulaziz Mannasahebhttps://orcid.org/0000-0003-2585-2018Mamdouh Saleh Alharbihttps://orcid.org/0009-0007-7998-0222Aejaz Abdullatif Khanhttps://orcid.org/0000-0002-5731-7522Salah Eldeen Dafallahttps://orcid.org/0000-0002-5022-2854S.M. Shakeel Iqubalhttps://orcid.org/0000-0001-5001-3537L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. This enzymatic process holds potential applications across diverse industries, notably in food and pharmaceuticals. The primary objective of the present research was to identify and isolate a fungal strain proficient in L-glutaminase production from soil found in maritime environments. The physical and nutritional conditions were optimized for maximum synthesis of L-glutaminase under solid state fermentation (SSF) and submerged fermentation conditions (SmF). The isolated organism was identified as Fusarium solani-melongenae strain CRI 24 by morphological and 18S rRNA analysis. The optimum carbon source under SmF and SSF was found to be starch (0.2% w/v). Wheat bran as solid substrate among others showed optimum enzyme activity. On the seventh day of incubation, at pH 8.0 and 0.7% L-glutamine concentration under SSF and SmF, the highest enzyme activity was detected. The greatest enzyme activity in SSF was seen at a moisture content of 10%. Fusarium solani-melongenae species produced the enzyme under optimal conditions and 4.20 and 4.73-fold increase (from 0.8 U/mL to 3.61 U/mL and from 0.781 U/mL to 3.69 U/mL) was achieved after optimization in submerged and in solid state fermentation, respectively. The selective isolation and optimization processes described in this work are a promising technique for the industrial production of L-glutaminase and can be applied in the pharmaceutical and food industries.https://microbiologyjournal.org/production-and-optimization-of-l-glutaminase-from-halophilic-fusarium-solani-melongenae-strain-cri-24-under-submerged-and-solid-state-fermentation/l-glutaminasefusarium solani-melongenaesolid state fermentationsubmerged fermentation |
| spellingShingle | M.S. Vineetha Nayef Abdulaziz Aldabaan Sunil S. More Mater H. Mahnashi Ibrahim Ahmed Shaikh Mohammad Shahzad Samdani Rashmi Swami Anirudh Yadav N. Rohith J. Bhavya Basheerahmed Abdulaziz Mannasaheb Mamdouh Saleh Alharbi Aejaz Abdullatif Khan Salah Eldeen Dafalla S.M. Shakeel Iqubal Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation Journal of Pure and Applied Microbiology l-glutaminase fusarium solani-melongenae solid state fermentation submerged fermentation |
| title | Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation |
| title_full | Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation |
| title_fullStr | Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation |
| title_full_unstemmed | Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation |
| title_short | Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation |
| title_sort | production and optimization of l glutaminase from halophilic fusarium solani melongenae strain cri 24 under submerged and solid state fermentation |
| topic | l-glutaminase fusarium solani-melongenae solid state fermentation submerged fermentation |
| url | https://microbiologyjournal.org/production-and-optimization-of-l-glutaminase-from-halophilic-fusarium-solani-melongenae-strain-cri-24-under-submerged-and-solid-state-fermentation/ |
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