Efficient large-scale preparation and purification of short single-stranded RNA oligonucleotides

Sequence-specific RNA recognition by RNA-binding proteins plays a crucial role in the post-translational regulation of gene expression. Biophysical and biochemical studies help to unravel the principles of sequence-specific RNA recognition, but the methods used require large amounts of single-strand...

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Bibliographic Details
Main Authors: Maria Zlobina, Ondrej Sedo, Ming-Yuan Chou, Lucia Slepankova, Peter J. Lukavsky
Format: Article
Language:English
Published: Taylor & Francis Group 2016-02-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114383