Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies
<p>Abstract</p> <p>Background</p> <p>Genomic aberrations can be used to determine cancer diagnosis and prognosis. Clinically relevant novel aberrations can be discovered using high-throughput assays such as Single Nucleotide Polymorphism (SNP) arrays and next-generation...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-05-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/230 |
| _version_ | 1823987948125683712 |
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| author | Xu Fang Cheng Elaine Hoyt Kathleen Bacchiocchi Antonella Narayan Deepak Ariyan Stephan Parisi Fabio Li Peining Halaban Ruth Kluger Yuval |
| author_facet | Xu Fang Cheng Elaine Hoyt Kathleen Bacchiocchi Antonella Narayan Deepak Ariyan Stephan Parisi Fabio Li Peining Halaban Ruth Kluger Yuval |
| author_sort | Xu Fang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Genomic aberrations can be used to determine cancer diagnosis and prognosis. Clinically relevant novel aberrations can be discovered using high-throughput assays such as Single Nucleotide Polymorphism (SNP) arrays and next-generation sequencing, which typically provide aggregate signals of many cells at once. However, heterogeneity of tumor subclones dramatically complicates the task of detecting aberrations.</p> <p>Results</p> <p>The aggregate signal of a population of subclones can be described as a linear system of equations. We employed a measure of allelic imbalance and total amount of DNA to characterize each locus by the copy number status (gain, loss or neither) of the strongest subclonal component. We designed simulated data to compare our measure to existing approaches and we analyzed SNP-arrays from 30 melanoma samples and transcriptome sequencing (RNA-Seq) from one melanoma sample.</p> <p>We showed that any system describing aggregate subclonal signals is underdetermined, leading to non-unique solutions for the exact copy number profile of subclones. For this reason, our illustrative measure was more robust than existing Hidden Markov Model (HMM) based tools in inferring the aberration status, as indicated by tests on simulated data. This higher robustness contributed in identifying numerous aberrations in several loci of melanoma samples. We validated the heterogeneity and aberration status within single biopsies by fluorescent <it>in situ </it>hybridization of four affected and transcriptionally up-regulated genes E2F8, ETV4, EZH2 and FAM84B in 11 melanoma cell lines. Heterogeneity was further demonstrated in the analysis of allelic imbalance changes along single exons from melanoma RNA-Seq.</p> <p>Conclusions</p> <p>These studies demonstrate how subclonal heterogeneity, prevalent in tumor samples, is reflected in aggregate signals measured by high-throughput techniques. Our proposed approach yields high robustness in detecting copy number alterations using high-throughput technologies and has the potential to identify specific subclonal markers from next-generation sequencing data.</p> |
| first_indexed | 2024-12-18T13:36:04Z |
| format | Article |
| id | doaj.art-cfaf9357a5574aeaa8e8941bdb329d8a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T13:36:04Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-cfaf9357a5574aeaa8e8941bdb329d8a2022-12-21T21:06:01ZengBMCBMC Genomics1471-21642011-05-0112123010.1186/1471-2164-12-230Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsiesXu FangCheng ElaineHoyt KathleenBacchiocchi AntonellaNarayan DeepakAriyan StephanParisi FabioLi PeiningHalaban RuthKluger Yuval<p>Abstract</p> <p>Background</p> <p>Genomic aberrations can be used to determine cancer diagnosis and prognosis. Clinically relevant novel aberrations can be discovered using high-throughput assays such as Single Nucleotide Polymorphism (SNP) arrays and next-generation sequencing, which typically provide aggregate signals of many cells at once. However, heterogeneity of tumor subclones dramatically complicates the task of detecting aberrations.</p> <p>Results</p> <p>The aggregate signal of a population of subclones can be described as a linear system of equations. We employed a measure of allelic imbalance and total amount of DNA to characterize each locus by the copy number status (gain, loss or neither) of the strongest subclonal component. We designed simulated data to compare our measure to existing approaches and we analyzed SNP-arrays from 30 melanoma samples and transcriptome sequencing (RNA-Seq) from one melanoma sample.</p> <p>We showed that any system describing aggregate subclonal signals is underdetermined, leading to non-unique solutions for the exact copy number profile of subclones. For this reason, our illustrative measure was more robust than existing Hidden Markov Model (HMM) based tools in inferring the aberration status, as indicated by tests on simulated data. This higher robustness contributed in identifying numerous aberrations in several loci of melanoma samples. We validated the heterogeneity and aberration status within single biopsies by fluorescent <it>in situ </it>hybridization of four affected and transcriptionally up-regulated genes E2F8, ETV4, EZH2 and FAM84B in 11 melanoma cell lines. Heterogeneity was further demonstrated in the analysis of allelic imbalance changes along single exons from melanoma RNA-Seq.</p> <p>Conclusions</p> <p>These studies demonstrate how subclonal heterogeneity, prevalent in tumor samples, is reflected in aggregate signals measured by high-throughput techniques. Our proposed approach yields high robustness in detecting copy number alterations using high-throughput technologies and has the potential to identify specific subclonal markers from next-generation sequencing data.</p>http://www.biomedcentral.com/1471-2164/12/230copy numberSNP arraysNext generation sequencingmelanoma |
| spellingShingle | Xu Fang Cheng Elaine Hoyt Kathleen Bacchiocchi Antonella Narayan Deepak Ariyan Stephan Parisi Fabio Li Peining Halaban Ruth Kluger Yuval Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies BMC Genomics copy number SNP arrays Next generation sequencing melanoma |
| title | Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| title_full | Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| title_fullStr | Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| title_full_unstemmed | Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| title_short | Detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| title_sort | detecting copy number status and uncovering subclonal markers in heterogeneous tumor biopsies |
| topic | copy number SNP arrays Next generation sequencing melanoma |
| url | http://www.biomedcentral.com/1471-2164/12/230 |
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