Establishment and application of a rapid assay for GII.4/GII.17 NoV detection based on the combination of CRISPR/Cas13a and isothermal amplification

Establishment and application of a rapid assay for GII.4/GII.17 NoV detection based on the combination of CRISPR/Cas13a and isothermal amplification

IntroductionNorovirus (NoV) is one of the most important agents responsible for viral acute gastroenteritis, among which GII.4 NoV is the predominant strain worldwide, and GII.17 NoV surpassed GII.4 in some epidemic seasons. Rapid and accurate gene recognition is essential for a timely response to N...

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Bibliographic Details
Main Authors: Jia-Heng Li, Duona Jing, Yu Wang, Jiayi Xu, Junxuan Yu, Huisha Du, Qing Chen, Shixing Tang, Xu-Fu Zhang, Ying-Chun Dai
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-02-01
Series:Frontiers in Microbiology
Subjects:
norovirus
genotype GII.4/GII.17
CRISPR/Cas13a
RAA
detection
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1334387/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2024.1334387/full

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