Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania
Abstract Plasmodium falciparum parasites lacking histidine-rich protein 2 and 3 (pfhrp2/3) genes have been reported in several parts of the world. These deletions are known to compromise the effectiveness of HRP2-based malaria rapid diagnostic tests (HRP2-RDT). The National Malaria Control Programme...
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09878-3 |
| _version_ | 1819045653429354496 |
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| author | Robert D. Kaaya Reginald A. Kavishe Filemon F. Tenu Johnson J. Matowo Franklin W. Mosha Chris Drakeley Colin J. Sutherland Khalid B. Beshir |
| author_facet | Robert D. Kaaya Reginald A. Kavishe Filemon F. Tenu Johnson J. Matowo Franklin W. Mosha Chris Drakeley Colin J. Sutherland Khalid B. Beshir |
| author_sort | Robert D. Kaaya |
| collection | DOAJ |
| description | Abstract Plasmodium falciparum parasites lacking histidine-rich protein 2 and 3 (pfhrp2/3) genes have been reported in several parts of the world. These deletions are known to compromise the effectiveness of HRP2-based malaria rapid diagnostic tests (HRP2-RDT). The National Malaria Control Programme (NMCP) in Tanzania adopted HRP2-RDTs as a routine tool for malaria diagnosis in 2009 replacing microscopy in many Health facilities. We investigated pfhrp2/3 deletions in 122 samples from two areas with diverse malaria transmission intensities in Northeastern Tanzania. Pfhrp2 deletion was confirmed in 1.6% of samples while pfhrp3 deletion was confirmed in 50% of samples. We did not find parasites with both pfhrp2 and pfhrp3 deletions among our samples. Results from this study highlight the need for systematic surveillance of pfhrp2/3 deletions in Tanzania to understand their prevalence and determine their impact on the performance of mRDT. |
| first_indexed | 2024-12-21T10:32:00Z |
| format | Article |
| id | doaj.art-cfcfb36beab94f82b127630c0af6af9b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T10:32:00Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cfcfb36beab94f82b127630c0af6af9b2022-12-21T19:07:10ZengNature PortfolioScientific Reports2045-23222022-04-011211810.1038/s41598-022-09878-3Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern TanzaniaRobert D. Kaaya0Reginald A. Kavishe1Filemon F. Tenu2Johnson J. Matowo3Franklin W. Mosha4Chris Drakeley5Colin J. Sutherland6Khalid B. Beshir7Kilimanjaro Christian Medical University CollegeKilimanjaro Christian Medical University CollegePan-African Malaria Vector Research ConsortiumKilimanjaro Christian Medical University CollegeKilimanjaro Christian Medical University CollegeDepartment of Infection Biology, Faculty of Infectious & Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious & Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious & Tropical Diseases, London School of Hygiene and Tropical MedicineAbstract Plasmodium falciparum parasites lacking histidine-rich protein 2 and 3 (pfhrp2/3) genes have been reported in several parts of the world. These deletions are known to compromise the effectiveness of HRP2-based malaria rapid diagnostic tests (HRP2-RDT). The National Malaria Control Programme (NMCP) in Tanzania adopted HRP2-RDTs as a routine tool for malaria diagnosis in 2009 replacing microscopy in many Health facilities. We investigated pfhrp2/3 deletions in 122 samples from two areas with diverse malaria transmission intensities in Northeastern Tanzania. Pfhrp2 deletion was confirmed in 1.6% of samples while pfhrp3 deletion was confirmed in 50% of samples. We did not find parasites with both pfhrp2 and pfhrp3 deletions among our samples. Results from this study highlight the need for systematic surveillance of pfhrp2/3 deletions in Tanzania to understand their prevalence and determine their impact on the performance of mRDT.https://doi.org/10.1038/s41598-022-09878-3 |
| spellingShingle | Robert D. Kaaya Reginald A. Kavishe Filemon F. Tenu Johnson J. Matowo Franklin W. Mosha Chris Drakeley Colin J. Sutherland Khalid B. Beshir Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania Scientific Reports |
| title | Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania |
| title_full | Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania |
| title_fullStr | Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania |
| title_full_unstemmed | Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania |
| title_short | Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania |
| title_sort | deletions of the plasmodium falciparum histidine rich protein 2 3 genes are common in field isolates from north eastern tanzania |
| url | https://doi.org/10.1038/s41598-022-09878-3 |
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