Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy
This review discusses various issues to consider when developing standard operating procedures for pre-clinical studies in the mdx mouse model of Duchenne muscular dystrophy (DMD). The review describes and evaluates a wide range of techniques used to measure parameters of muscle pathology in mdx mic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008-07-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S096999610800065X |
| _version_ | 1818862607424028672 |
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| author | Miranda D. Grounds Hannah G. Radley Gordon S. Lynch Kanneboyina Nagaraju Annamaria De Luca |
| author_facet | Miranda D. Grounds Hannah G. Radley Gordon S. Lynch Kanneboyina Nagaraju Annamaria De Luca |
| author_sort | Miranda D. Grounds |
| collection | DOAJ |
| description | This review discusses various issues to consider when developing standard operating procedures for pre-clinical studies in the mdx mouse model of Duchenne muscular dystrophy (DMD). The review describes and evaluates a wide range of techniques used to measure parameters of muscle pathology in mdx mice and identifies some basic techniques that might comprise standardised approaches for evaluation. While the central aim is to provide a basis for the development of standardised procedures to evaluate efficacy of a drug or a therapeutic strategy, a further aim is to gain insight into pathophysiological mechanisms in order to identify other therapeutic targets. The desired outcome is to enable easier and more rigorous comparison of pre-clinical data from different laboratories around the world, in order to accelerate identification of the best pre-clinical therapies in the mdx mouse that will fast-track translation into effective clinical treatments for DMD. |
| first_indexed | 2024-12-19T10:02:33Z |
| format | Article |
| id | doaj.art-cfd182d0925842dd818445cac43fe7c5 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-19T10:02:33Z |
| publishDate | 2008-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-cfd182d0925842dd818445cac43fe7c52022-12-21T20:26:36ZengElsevierNeurobiology of Disease1095-953X2008-07-01311119Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophyMiranda D. Grounds0Hannah G. Radley1Gordon S. Lynch2Kanneboyina Nagaraju3Annamaria De Luca4School of Anatomy and Human Biology, the University of Western Australia, Perth, Western Australia, Australia; Corresponding author. Fax: +61 8 6488 1051.School of Anatomy and Human Biology, the University of Western Australia, Perth, Western Australia, AustraliaBasic and Clinical Myology Laboratory, Department of Physiology, the University of Melbourne, Victoria, AustraliaResearch Centre for Genetic Medicine, Murine Drug Testing Facility, Children's National Medical Center, Washington, USASezione di Farmacologia, Dipartimento Farmacobiologico, Facoltà di Farmacia, Università di Bari, Bari, ItalyThis review discusses various issues to consider when developing standard operating procedures for pre-clinical studies in the mdx mouse model of Duchenne muscular dystrophy (DMD). The review describes and evaluates a wide range of techniques used to measure parameters of muscle pathology in mdx mice and identifies some basic techniques that might comprise standardised approaches for evaluation. While the central aim is to provide a basis for the development of standardised procedures to evaluate efficacy of a drug or a therapeutic strategy, a further aim is to gain insight into pathophysiological mechanisms in order to identify other therapeutic targets. The desired outcome is to enable easier and more rigorous comparison of pre-clinical data from different laboratories around the world, in order to accelerate identification of the best pre-clinical therapies in the mdx mouse that will fast-track translation into effective clinical treatments for DMD.http://www.sciencedirect.com/science/article/pii/S096999610800065XMdx mouseMuscular dystrophyStandard operating proceduresBiological variationMuscle functionPre-clinical trials |
| spellingShingle | Miranda D. Grounds Hannah G. Radley Gordon S. Lynch Kanneboyina Nagaraju Annamaria De Luca Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy Neurobiology of Disease Mdx mouse Muscular dystrophy Standard operating procedures Biological variation Muscle function Pre-clinical trials |
| title | Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy |
| title_full | Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy |
| title_fullStr | Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy |
| title_full_unstemmed | Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy |
| title_short | Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy |
| title_sort | towards developing standard operating procedures for pre clinical testing in the mdx mouse model of duchenne muscular dystrophy |
| topic | Mdx mouse Muscular dystrophy Standard operating procedures Biological variation Muscle function Pre-clinical trials |
| url | http://www.sciencedirect.com/science/article/pii/S096999610800065X |
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