Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP
GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, toge...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2018-04-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=2795&type=0 |