Single-Molecule Localization Microscopy of Presynaptic Active Zones in <i>Drosophila melanogaster</i> after Rapid Cryofixation

Single-Molecule Localization Microscopy of Presynaptic Active Zones in <i>Drosophila melanogaster</i> after Rapid Cryofixation

Single-molecule localization microscopy (SMLM) greatly advances structural studies of diverse biological tissues. For example, presynaptic active zone (AZ) nanotopology is resolved in increasing detail. Immunofluorescence imaging of AZ proteins usually relies on epitope preservation using aldehyde-b...

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Bibliographic Details
Main Authors: Achmed Mrestani, Katharina Lichter, Anna-Leena Sirén, Manfred Heckmann, Mila M. Paul, Martin Pauli
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:International Journal of Molecular Sciences
Subjects:
active zone
nanotopology
neuromuscular junction
high-pressure freezing/freeze substitution
PFA in ethanol
<i>d</i>STORM
Online Access:https://www.mdpi.com/1422-0067/24/3/2128

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https://www.mdpi.com/1422-0067/24/3/2128

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