Partial purification of crude lipase extract from Yarrowia lipolytica: Precipitation, aqueous two-phase systems (ATPS), and immobilization methods

Partial purification of crude lipase extract from Yarrowia lipolytica: Precipitation, aqueous two-phase systems (ATPS), and immobilization methods

Efforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; a...

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Bibliographic Details
Main Authors: Ana I.S. Brígida, Filipe S. Buarque, Vanessa L.R. Nogueira, Vânia M.M. Melo, Jose M. Guisán, Bernardo D. Ribeiro, Luciana R.B. Gonçalves, Maria A.Z. Coelho
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Cleaner Chemical Engineering
Subjects:
Y. lipolytica IMUFRJ 50682
Purification
Precipitation
ATPS
Immobilization
Online Access:http://www.sciencedirect.com/science/article/pii/S277278232300013X

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http://www.sciencedirect.com/science/article/pii/S277278232300013X

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