IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer
AbstractVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cance...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2242419 |
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| author | Asad Syed Abu Baker Mohamed Mohany Abdallah M. Elgorban Mohd Sajid Khan Salim S. Al-Rejaie |
| author_facet | Asad Syed Abu Baker Mohamed Mohany Abdallah M. Elgorban Mohd Sajid Khan Salim S. Al-Rejaie |
| author_sort | Asad Syed |
| collection | DOAJ |
| description | AbstractVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibody-dependent cellular cytotoxicity and macrophage-mediated antibody-dependent phagocytosis, respectively. Further, IgGAuNPs and MTX-IgGAuNPs were characterised by physical techniques. Moreover, 3D conformational changes in the structure of IgG were analysed by fluorescence spectroscopy during and after the synthesis of IgGAuNPs. Furthermore, the IgGAuNPs and MTX-IgGAuNPs were effective against lung cancer (A549 cells), while they were found to be non-toxic against normal cells (NRK cells). The effectiveness of IgGAuNPs and MTX-IgGAuNPs was examined by MTT cytotoxicity assay, DCFDA method for the production of ROS, and release of Cyt-c from the mitochondria for caspase-3-mediated apoptosis. Moreover, the confirmation of internalisation of particles into the nucleus was examined under the DAPI assay, and it was found that particles caused nuclear fragmentation, which was also an indication of apoptosis. |
| first_indexed | 2024-03-11T11:17:53Z |
| format | Article |
| id | doaj.art-d0093eebc59847cb9e2eb8f4519e0b68 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:17:53Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-d0093eebc59847cb9e2eb8f4519e0b682023-11-10T17:19:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151138439610.1080/21691401.2023.2242419IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancerAsad Syed0Abu Baker1Mohamed Mohany2Abdallah M. Elgorban3Mohd Sajid Khan4Salim S. Al-Rejaie5Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi ArabiaNanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026 IndiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. 55760, Riyadh 11451, Saudi ArabiaCenter of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi ArabiaNanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026 IndiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. 55760, Riyadh 11451, Saudi ArabiaAbstractVincamine, a natural chemical, was used as a reducing agent in the synthesis of IgG antibodies mediated biogenic gold nanoparticles (IgGAuNPs). Eventually, the synthesised IgGAuNPs were bioconjugated with the chemotherapeutic drug methotrexate (MTX-IgGAuNPs). The IgG isotype can target cancer cells through polymorphic Fc gamma receptors (FcγRs) and have therapeutic effects. They can restrict cell division by inhibiting different intracellular signal transduction pathways and activating NK cells and macrophages through antibody-dependent cellular cytotoxicity and macrophage-mediated antibody-dependent phagocytosis, respectively. Further, IgGAuNPs and MTX-IgGAuNPs were characterised by physical techniques. Moreover, 3D conformational changes in the structure of IgG were analysed by fluorescence spectroscopy during and after the synthesis of IgGAuNPs. Furthermore, the IgGAuNPs and MTX-IgGAuNPs were effective against lung cancer (A549 cells), while they were found to be non-toxic against normal cells (NRK cells). The effectiveness of IgGAuNPs and MTX-IgGAuNPs was examined by MTT cytotoxicity assay, DCFDA method for the production of ROS, and release of Cyt-c from the mitochondria for caspase-3-mediated apoptosis. Moreover, the confirmation of internalisation of particles into the nucleus was examined under the DAPI assay, and it was found that particles caused nuclear fragmentation, which was also an indication of apoptosis.https://www.tandfonline.com/doi/10.1080/21691401.2023.2242419Gold nanoparticlesIgG antibodiesmethotrexatebioconjugationlung cancer |
| spellingShingle | Asad Syed Abu Baker Mohamed Mohany Abdallah M. Elgorban Mohd Sajid Khan Salim S. Al-Rejaie IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer Artificial Cells, Nanomedicine, and Biotechnology Gold nanoparticles IgG antibodies methotrexate bioconjugation lung cancer |
| title | IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| title_full | IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| title_fullStr | IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| title_full_unstemmed | IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| title_short | IgG antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| title_sort | igg antibodies mediated gold nanoparticles conjugated to methotrexate as targeted chemotherapy for lung cancer |
| topic | Gold nanoparticles IgG antibodies methotrexate bioconjugation lung cancer |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2242419 |
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