Fluoride-Controlled Riboswitch-Based Dampening of Gene Expression for Cloning Potent Promoters

Bioreporter systems based on detectable enzyme activity, such as that of beta-galactosidase or luciferase, are key in novel bacterial promoter discovery and study. While these systems permit quantification of gene expression, their use is limited by the toxicity of the expressed reporter enzymes in...

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Bibliographic Details
Main Authors: Vesta Korniakova, Aurélie Devinck, Marie-Christine Groleau, Eric Déziel, Jonathan Perreault
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Genetics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.591543/full