Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils
<i>Hodgsonia heteroclita</i> subsp. <i>Indochinensis</i> W.J.de Wlide & Duyfjes (or Making in Thai) is a neglected and underutilized crop (NUC) with high fat containing nuts. In this study, the enzyme-assisted mechanical extraction of <i>H. heteroclita</i> see...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/2/292 |
| _version_ | 1797442596891525120 |
|---|---|
| author | Jirachaya Piseskul Uthaiwan Suttisansanee Chaowanee Chupeerach Chanakan Khemthong Sirinapa Thangsiri Piya Temviriyanukul Yuraporn Sahasakul Chalat Santivarangkna Rungrat Chamchan Amornrat Aursalung Nattira On–nom |
| author_facet | Jirachaya Piseskul Uthaiwan Suttisansanee Chaowanee Chupeerach Chanakan Khemthong Sirinapa Thangsiri Piya Temviriyanukul Yuraporn Sahasakul Chalat Santivarangkna Rungrat Chamchan Amornrat Aursalung Nattira On–nom |
| author_sort | Jirachaya Piseskul |
| collection | DOAJ |
| description | <i>Hodgsonia heteroclita</i> subsp. <i>Indochinensis</i> W.J.de Wlide & Duyfjes (or Making in Thai) is a neglected and underutilized crop (NUC) with high fat containing nuts. In this study, the enzyme-assisted mechanical extraction of <i>H. heteroclita</i> seed oil was investigated using response surface methodology (RSM) to predict the optimal fat extraction conditions. The most efficient enzyme used in the experiment was a mixture of Flavourzyme<sup>®</sup> and Viscozyme<sup>®</sup> (1:1, <i>w</i>/<i>w</i>). The predicted maximum oil yield was 46.44%, using the following extraction conditions: 2.98% (<i>w</i>/<i>w</i>) enzyme loading, 48 °C incubation temperature and 76 min of incubation time. <i>H. heteroclita</i> seed oil obtained from heat and enzymatic pretreatments exhibited the highest lightness and viscosity. The chemical properties of this seed oil, including water and volatile compounds (≤0.2% <i>w</i>/<i>w</i>), acid value (≤4.0 mg KOH/g), peroxide value (≤15 mEq of active oxygen/kg) and soap content (≤0.005% <i>w</i>/<i>w</i>), were within the acceptable levels specified by the Codex Alimentarius (2019). <i>H. heteroclita</i> seed oil obtained from heat and enzymatic pretreatments contained the highest content of δ-tocopherol (88.29 mg/100 g) and omega-6 fatty acids (48.19 g/100 g). This study is the first to report on the enzyme-assisted mechanical extraction of <i>H. heteroclita</i> oilseeds as a promising plant material for vegetable oil production. |
| first_indexed | 2024-03-09T12:44:15Z |
| format | Article |
| id | doaj.art-d00f990704864443b3213b71bb44ed7b |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:44:15Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d00f990704864443b3213b71bb44ed7b2023-11-30T22:14:22ZengMDPI AGFoods2304-81582023-01-0112229210.3390/foods12020292Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the OilsJirachaya Piseskul0Uthaiwan Suttisansanee1Chaowanee Chupeerach2Chanakan Khemthong3Sirinapa Thangsiri4Piya Temviriyanukul5Yuraporn Sahasakul6Chalat Santivarangkna7Rungrat Chamchan8Amornrat Aursalung9Nattira On–nom10Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand<i>Hodgsonia heteroclita</i> subsp. <i>Indochinensis</i> W.J.de Wlide & Duyfjes (or Making in Thai) is a neglected and underutilized crop (NUC) with high fat containing nuts. In this study, the enzyme-assisted mechanical extraction of <i>H. heteroclita</i> seed oil was investigated using response surface methodology (RSM) to predict the optimal fat extraction conditions. The most efficient enzyme used in the experiment was a mixture of Flavourzyme<sup>®</sup> and Viscozyme<sup>®</sup> (1:1, <i>w</i>/<i>w</i>). The predicted maximum oil yield was 46.44%, using the following extraction conditions: 2.98% (<i>w</i>/<i>w</i>) enzyme loading, 48 °C incubation temperature and 76 min of incubation time. <i>H. heteroclita</i> seed oil obtained from heat and enzymatic pretreatments exhibited the highest lightness and viscosity. The chemical properties of this seed oil, including water and volatile compounds (≤0.2% <i>w</i>/<i>w</i>), acid value (≤4.0 mg KOH/g), peroxide value (≤15 mEq of active oxygen/kg) and soap content (≤0.005% <i>w</i>/<i>w</i>), were within the acceptable levels specified by the Codex Alimentarius (2019). <i>H. heteroclita</i> seed oil obtained from heat and enzymatic pretreatments contained the highest content of δ-tocopherol (88.29 mg/100 g) and omega-6 fatty acids (48.19 g/100 g). This study is the first to report on the enzyme-assisted mechanical extraction of <i>H. heteroclita</i> oilseeds as a promising plant material for vegetable oil production.https://www.mdpi.com/2304-8158/12/2/292pretreatmentseed oilresponse surface methodologyfatty acid profileneglected and underutilized cropssustainability |
| spellingShingle | Jirachaya Piseskul Uthaiwan Suttisansanee Chaowanee Chupeerach Chanakan Khemthong Sirinapa Thangsiri Piya Temviriyanukul Yuraporn Sahasakul Chalat Santivarangkna Rungrat Chamchan Amornrat Aursalung Nattira On–nom Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils Foods pretreatment seed oil response surface methodology fatty acid profile neglected and underutilized crops sustainability |
| title | Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils |
| title_full | Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils |
| title_fullStr | Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils |
| title_full_unstemmed | Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils |
| title_short | Optimization of Enzyme-Assisted Mechanical Extraction Process of <i>Hodgsonia heteroclita</i> Oilseeds and Physical, Chemical, and Nutritional Properties of the Oils |
| title_sort | optimization of enzyme assisted mechanical extraction process of i hodgsonia heteroclita i oilseeds and physical chemical and nutritional properties of the oils |
| topic | pretreatment seed oil response surface methodology fatty acid profile neglected and underutilized crops sustainability |
| url | https://www.mdpi.com/2304-8158/12/2/292 |
| work_keys_str_mv | AT jirachayapiseskul optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT uthaiwansuttisansanee optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT chaowaneechupeerach optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT chanakankhemthong optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT sirinapathangsiri optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT piyatemviriyanukul optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT yurapornsahasakul optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT chalatsantivarangkna optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT rungratchamchan optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT amornrataursalung optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils AT nattiraonnom optimizationofenzymeassistedmechanicalextractionprocessofihodgsoniaheteroclitaioilseedsandphysicalchemicalandnutritionalpropertiesoftheoils |