CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing

CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing

ABSTRACT Shigella is a Gram-negative bacterium that invades the human gut epithelium. The resulting infection, shigellosis, is the deadliest bacterial diarrheal disease. Much of the information about the genes dictating the pathophysiology of Shigella, both on the chromosome and the virulence plasmi...

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Bibliographic Details
Main Authors: Atin Sharma, Ruqiya Omer Aden, Andrea Puhar, David A. Cisneros
Format: Article
Language:English
Published: American Society for Microbiology 2023-02-01
Series:mSystems
Subjects:
Enterobacteriaceae
loss of function mutagenesis
polar effects
polar mutagenesis
Online Access:https://journals.asm.org/doi/10.1128/msystems.01045-22

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https://journals.asm.org/doi/10.1128/msystems.01045-22

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