Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm of Bacillus subtilis

Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm of Bacillus subtilis

The influence of fusion tags to produce recombinant proteins in the cytoplasm of Bacillus subtilis is not well-studied as in E. coli. This study aimed to investigate the influence of His-tags with different codons on the protein production levels of the high expression gene (gfp+) and low expression...

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Bibliographic Details
Main Authors: Ngan Thi Phuong Le, Trang Thi Phuong Phan, Hanh Thi Thu Phan, Tuom Thi Tinh Truong, Wolfgang Schumann, Hoang Duc Nguyen
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Biotechnology Reports
Subjects:
His-tag
Fusion tag
Pgrac212 promoter
Bacillus subtilis
Low expression gene
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X22000522

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http://www.sciencedirect.com/science/article/pii/S2215017X22000522

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