Finding a needle in a haystack: identification of EGFP tagged neurons during Calcium imaging by means of two-photon spectral separation.

Finding a needle in a haystack: identification of EGFP tagged neurons during Calcium imaging by means of two-photon spectral separation.

The combination of two-photon in vivo imaging and genetic labeling of specific cell types in the mouse brain is a powerful method to refine our understanding of brain circuitry and to dissect the contribution of specific neural classes to cortical function. The synthetic calcium indicators are the b...

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Bibliographic Details
Main Authors: Marco eBrondi, Sebastian eSulis Sato, Luigi Federico eRossi, Silvia eFerrara, Gian Michele eRatto
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-10-01
Series:Frontiers in Molecular Neuroscience
Subjects:
spectroscopy
EGFP
Oregon Green BAPTA 1
In vivo targeted two photon microscopy
scattering
in vivo functional imaging
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00096/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00096/full

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