Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs
We analyzed transcriptomic data from otic sensory cells differentiated from human induced pluripotent stem cells (hiPSCs) by a previously described method to gain new insights into the early human otic neurosensory lineage. We identified genes and biological networks not previously described to occu...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10849 |
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| author | Lejo Johnson Chacko Hanae Lahlou Claudia Steinacher Said Assou Yassine Messat József Dudás Albert Edge Berta Crespo Moira Crosier Consolato Sergi Anneliese Schrott-Fischer Azel Zine |
| author_facet | Lejo Johnson Chacko Hanae Lahlou Claudia Steinacher Said Assou Yassine Messat József Dudás Albert Edge Berta Crespo Moira Crosier Consolato Sergi Anneliese Schrott-Fischer Azel Zine |
| author_sort | Lejo Johnson Chacko |
| collection | DOAJ |
| description | We analyzed transcriptomic data from otic sensory cells differentiated from human induced pluripotent stem cells (hiPSCs) by a previously described method to gain new insights into the early human otic neurosensory lineage. We identified genes and biological networks not previously described to occur in the human otic sensory developmental cell lineage. These analyses identified and ranked genes known to be part of the otic sensory lineage program (SIX1, EYA1, GATA3, etc.), in addition to a number of novel genes encoding extracellular matrix (ECM) (COL3A1, COL5A2, DCN, etc.) and integrin (ITG) receptors (ITGAV, ITGA4, ITGA) for ECM molecules. The results were confirmed by quantitative PCR analysis of a comprehensive panel of genes differentially expressed during the time course of hiPSC differentiation in vitro. Immunocytochemistry validated results for select otic and ECM/ITG gene markers in the in vivo human fetal inner ear. Our screen shows ECM and ITG gene expression changes coincident with hiPSC differentiation towards human otic neurosensory cells. Our findings suggest a critical role of ECM-ITG interactions with otic neurosensory lineage genes in early neurosensory development and cell fate determination in the human fetal inner ear. |
| first_indexed | 2024-03-10T06:59:17Z |
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| id | doaj.art-d084fb36f922445c9a2cfb599abbba5c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:59:17Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d084fb36f922445c9a2cfb599abbba5c2023-11-22T16:16:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122191084910.3390/ijms221910849Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCsLejo Johnson Chacko0Hanae Lahlou1Claudia Steinacher2Said Assou3Yassine Messat4József Dudás5Albert Edge6Berta Crespo7Moira Crosier8Consolato Sergi9Anneliese Schrott-Fischer10Azel Zine11Inner Ear Laboratory, Department of Otorhinolaryngology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaHarvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02101, USAInner Ear Laboratory, Department of Otorhinolaryngology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaIRMB, Univ Montpellier, INSERM, CHU Montpellier, 34000 Montpellier, FranceLBN, Laboratory of Bioengineering and Nanoscience, Univ Montpellier, 34090 Montpellier, FranceInner Ear Laboratory, Department of Otorhinolaryngology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaHarvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02101, USAUCL Great Ormond Street Institute of Child Health, University College London, London WC1 N1EH, UKHuman Development Biology Resource, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle NE1 4EP, UKAnatomic Pathology Division, Children’s Hospital of Eastern Ontario, University of Ottawa, Ottawa, ON K1H 8L1, CanadaInner Ear Laboratory, Department of Otorhinolaryngology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, AustriaLBN, Laboratory of Bioengineering and Nanoscience, Univ Montpellier, 34090 Montpellier, FranceWe analyzed transcriptomic data from otic sensory cells differentiated from human induced pluripotent stem cells (hiPSCs) by a previously described method to gain new insights into the early human otic neurosensory lineage. We identified genes and biological networks not previously described to occur in the human otic sensory developmental cell lineage. These analyses identified and ranked genes known to be part of the otic sensory lineage program (SIX1, EYA1, GATA3, etc.), in addition to a number of novel genes encoding extracellular matrix (ECM) (COL3A1, COL5A2, DCN, etc.) and integrin (ITG) receptors (ITGAV, ITGA4, ITGA) for ECM molecules. The results were confirmed by quantitative PCR analysis of a comprehensive panel of genes differentially expressed during the time course of hiPSC differentiation in vitro. Immunocytochemistry validated results for select otic and ECM/ITG gene markers in the in vivo human fetal inner ear. Our screen shows ECM and ITG gene expression changes coincident with hiPSC differentiation towards human otic neurosensory cells. Our findings suggest a critical role of ECM-ITG interactions with otic neurosensory lineage genes in early neurosensory development and cell fate determination in the human fetal inner ear.https://www.mdpi.com/1422-0067/22/19/10849human induced pluripotent stem cellsotic sensory progenitorsgene expression analysisextracellular matrixintegrinshuman fetal inner ear |
| spellingShingle | Lejo Johnson Chacko Hanae Lahlou Claudia Steinacher Said Assou Yassine Messat József Dudás Albert Edge Berta Crespo Moira Crosier Consolato Sergi Anneliese Schrott-Fischer Azel Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences human induced pluripotent stem cells otic sensory progenitors gene expression analysis extracellular matrix integrins human fetal inner ear |
| title | Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs |
| title_full | Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs |
| title_fullStr | Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs |
| title_full_unstemmed | Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs |
| title_short | Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs |
| title_sort | transcriptome wide analysis reveals a role for extracellular matrix and integrin receptor genes in otic neurosensory differentiation from human ipscs |
| topic | human induced pluripotent stem cells otic sensory progenitors gene expression analysis extracellular matrix integrins human fetal inner ear |
| url | https://www.mdpi.com/1422-0067/22/19/10849 |
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