Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition
AbstractClodronate liposomes are bisphosphonates encapsulated by liposomes that are known to induce macrophage depletion in vivo. In a previous study, clodronate liposomes improved renal ischemia/reperfusion (I/R) injury in mice, which may be due to effects on macrophage phenotypes. However, how inf...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2022.2149412 |
| _version_ | 1797658463983108096 |
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| author | Zhizhi Hu Juan Zhan Guangchang Pei Rui Zeng |
| author_facet | Zhizhi Hu Juan Zhan Guangchang Pei Rui Zeng |
| author_sort | Zhizhi Hu |
| collection | DOAJ |
| description | AbstractClodronate liposomes are bisphosphonates encapsulated by liposomes that are known to induce macrophage depletion in vivo. In a previous study, clodronate liposomes improved renal ischemia/reperfusion (I/R) injury in mice, which may be due to effects on macrophage phenotypes. However, how inflammatory cytokines secretion participates is unknown. In this study, we investigated the effect of macrophages in the I/R kidney by depleting macrophages with clodronate liposomes and changing inflammatory cytokines. C57BL/6 mice underwent I/R injury with or without clodronate liposomes administration on Days 5 and 15. Tubular injury, collagen deposition, and fibrosis were detected and analyzed by histological staining, immunocytochemistry (IHC), flow cytometry (FACS), and reverse transcription–polymerase chain reaction (RT–PCR). Inflammatory cytokines were detected and analyzed by Western blotting and RT–PCR. We found that clodronate liposomes alleviated renal fibrosis and tissue damage on both Days 5 and 15. KIM-1, IL-10, and TGF-β were reduced significantly in the clodronate liposomes treatment group. However, TNF-α was not different between the clodronate liposomes treatment group and the phosphate-buffered saline treatment group on either Day 5 or Day 15. Thus, clodronate liposomes can alleviate renal fibrosis and tissue damage and reduce the inflammatory cytokines IL-10 and TGF-β, suggesting that clodronate liposomes alleviate renal fibrosis may because of M1/M2 polarization. |
| first_indexed | 2024-03-11T18:00:26Z |
| format | Article |
| id | doaj.art-d08922ea87694bbcb564d0265624ad24 |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-03-11T18:00:26Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-d08922ea87694bbcb564d0265624ad242023-10-17T09:23:24ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492023-12-0145110.1080/0886022X.2022.2149412Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transitionZhizhi Hu0Juan Zhan1Guangchang Pei2Rui Zeng3Division of Nephrology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, People’s Republic of ChinaDivision of Nephrology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, People’s Republic of ChinaDivision of Nephrology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, People’s Republic of ChinaDivision of Nephrology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, People’s Republic of ChinaAbstractClodronate liposomes are bisphosphonates encapsulated by liposomes that are known to induce macrophage depletion in vivo. In a previous study, clodronate liposomes improved renal ischemia/reperfusion (I/R) injury in mice, which may be due to effects on macrophage phenotypes. However, how inflammatory cytokines secretion participates is unknown. In this study, we investigated the effect of macrophages in the I/R kidney by depleting macrophages with clodronate liposomes and changing inflammatory cytokines. C57BL/6 mice underwent I/R injury with or without clodronate liposomes administration on Days 5 and 15. Tubular injury, collagen deposition, and fibrosis were detected and analyzed by histological staining, immunocytochemistry (IHC), flow cytometry (FACS), and reverse transcription–polymerase chain reaction (RT–PCR). Inflammatory cytokines were detected and analyzed by Western blotting and RT–PCR. We found that clodronate liposomes alleviated renal fibrosis and tissue damage on both Days 5 and 15. KIM-1, IL-10, and TGF-β were reduced significantly in the clodronate liposomes treatment group. However, TNF-α was not different between the clodronate liposomes treatment group and the phosphate-buffered saline treatment group on either Day 5 or Day 15. Thus, clodronate liposomes can alleviate renal fibrosis and tissue damage and reduce the inflammatory cytokines IL-10 and TGF-β, suggesting that clodronate liposomes alleviate renal fibrosis may because of M1/M2 polarization.https://www.tandfonline.com/doi/10.1080/0886022X.2022.2149412Ischemia/reperfusion injurymacrophagerenal fibrosisclodronate liposomes |
| spellingShingle | Zhizhi Hu Juan Zhan Guangchang Pei Rui Zeng Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition Renal Failure Ischemia/reperfusion injury macrophage renal fibrosis clodronate liposomes |
| title | Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition |
| title_full | Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition |
| title_fullStr | Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition |
| title_full_unstemmed | Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition |
| title_short | Depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on AKI–CKD transition |
| title_sort | depletion of macrophages with clodronate liposomes partially attenuates renal fibrosis on aki ckd transition |
| topic | Ischemia/reperfusion injury macrophage renal fibrosis clodronate liposomes |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2022.2149412 |
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