Insights into glycosidic bond specificity of an engineered selective α-L-rhamnosidase N12-Rha via activity assays and molecular modelling

Insights into glycosidic bond specificity of an engineered selective α-L-rhamnosidase N12-Rha via activity assays and molecular modelling

Abstract αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Aspergillus niger JMU-TS528 had significantly higher catalytic activity on α-1,6 glycosidic bond than α-1,2 glycosidic bond, and had no activit...

Full description

Bibliographic Details
Main Authors: Bo Yu, Shiyu Luo, Yuhan Ding, Zijie Gong, Ting Nie
Format: Article
Language:English
Published: SpringerOpen 2022-11-01
Series:AMB Express
Subjects:
α-L-rhamnosidase
Catalytic activity
Charge difference
Online Access:https://doi.org/10.1186/s13568-022-01489-5

Internet

https://doi.org/10.1186/s13568-022-01489-5

Similar Items