Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

Summary: Most of the protocols to analyze metabolic features of cell populations from different tissues rely on in vitro cell culture conditions. Here, we present a flow-cytometry-based protocol for measuring the respiratory chain function in permeabilized mouse microglia ex vivo. We describe microg...

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Bibliographic Details
Main Authors: Daniel Erny, Nikolaos Dokalis, Charlotte Mezö, Omar Mossad, Thomas Blank, Marco Prinz
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Metabolism
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000661

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http://www.sciencedirect.com/science/article/pii/S2666166722000661

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