A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol

A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol

Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [3H]cholesterol and measuring release of the labeled sterol. Using [3H]cholesterol is not ideal for the...

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Bibliographic Details
Main Authors: Sandhya Sankaranarayanan, Ginny Kellner-Weibel, Margarita de la Llera-Moya, Michael C. Phillips, Bela F. Asztalos, Robert Bittman, George H. Rothblat
Format: Article
Language:English
Published: Elsevier 2011-12-01
Series:Journal of Lipid Research
Subjects:
BODIPY-cholesterol (boron dipyrromethene difluoride linked to sterol carbon -24) efflux
ABCA1
coronary artery disease
atherosclerosis
apo A-I
apoB-depleted serum
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520408387

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http://www.sciencedirect.com/science/article/pii/S0022227520408387

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