A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol

A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol

Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [3H]cholesterol and measuring release of the labeled sterol. Using [3H]cholesterol is not ideal for the...

Full description

Bibliographic Details
Main Authors:	Sandhya Sankaranarayanan, Ginny Kellner-Weibel, Margarita de la Llera-Moya, Michael C. Phillips, Bela F. Asztalos, Robert Bittman, George H. Rothblat
Format:	Article
Language:	English
Published:	Elsevier 2011-12-01
Series:	Journal of Lipid Research
Subjects:	BODIPY-cholesterol (boron dipyrromethene difluoride linked to sterol carbon -24) efflux ABCA1 coronary artery disease atherosclerosis apo A-I apoB-depleted serum
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520408387

Similar Items

Effect of 1-deoxynojirimycin on cholesterol efflux through ABCA1-LXRα pathway in 3T3-L1 adipocytes
by: Hyun Ju Do, et al.
Published: (2014-03-01)

Dipeptidyl Peptidase-4 Inhibitor Sitagliptin Phosphate Accelerates Cellular Cholesterol Efflux in THP-1 Cells
by: Tomohiro Komatsu, et al.
Published: (2023-01-01)

An apolipoprotein B antisense oligonucleotide lowers LDL cholesterol in hyperlipidemic mice without causing hepatic steatosis
by: Rosanne M. Crooke, et al.
Published: (2005-05-01)

Differences in apolipoprotein and lipid composition between human chylomicron remnants and very low density lipoproteins isolated from fasting and postprandial plasma
by: Johan Björkegren, et al.
Published: (1998-07-01)

Postprandial decrease in LDL-cholesterol in men with metabolic syndrome
by: Skoczyńska Anna, et al.
Published: (2015-02-01)

The association of apolipoprotein in the risk of ST-elevation myocardial infarction in patients with documented coronary artery disease
by: Astuti Giantini, et al.
Published: (2023-09-01)

Standardization of Apolipoprotein B, LDL‐Cholesterol, and Non‐HDL‐Cholesterol
by: John H. Contois, et al.
Published: (2023-08-01)

Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly
by: Emile Levy, et al.
Published: (2000-01-01)

Testing the role of apoA-I, HDL, and cholesterol efflux in the atheroprotective action of low-level apoE expression
by: Fayanne E. Thorngate, et al.
Published: (2003-12-01)

ApoB100 and Atherosclerosis: What’s New in the 21st Century?
by: Dimitris Kounatidis, et al.
Published: (2024-02-01)

The N-linked oligosaccharides at the amino terminus of human apoB are important for the assembly and secretion of VLDL
by: Jelena Vukmirica, et al.
Published: (2002-09-01)

HDL, cholesterol efflux, and ABCA1: Free from good and evil dualism
by: Masatsune Ogura
Published: (2022-10-01)

Assembly of very low density lipoproteins in mouse liver: evidence of heterogeneity of particle density in the Golgi apparatus
by: Larry L. Swift, et al.
Published: (2001-02-01)

Importance of macrophage cholesterol content on the flux of cholesterol mass
by: Sandhya Sankaranarayanan, et al.
Published: (2010-11-01)

Is reverse cholesterol transport regulated by active cholesterol?
by: Theodore L. Steck, et al.
Published: (2023-06-01)

Functional characterization of missense variants affecting the extracellular domains of ABCA1 using a fluorescence-based assay
by: Marianne Teigen, et al.
Published: (2024-01-01)

Physiological Bases for the Superiority of Apolipoprotein B Over Low‐Density Lipoprotein Cholesterol and Non–High‐Density Lipoprotein Cholesterol as a Marker of Cardiovascular Risk
by: Tamara Glavinovic, et al.
Published: (2022-10-01)

Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition
by: Shifa Jebari-Benslaiman, et al.
Published: (2020-09-01)

ABCA6 Regulates Chondrogenesis and Inhibits Joint Degeneration via Orchestrated Cholesterol Efflux and Cellular Senescence
by: Yi Wang, et al.
Published: (2025-03-01)

ABCA1‐Mediated Cholesterol Efflux Capacity to Cerebrospinal Fluid Is Reduced in Patients With Mild Cognitive Impairment and Alzheimer's Disease
by: Hussein N. Yassine, et al.
Published: (2016-02-01)

Inflammatory corpuscle AIM2 facilitates macrophage foam cell formation by inhibiting cholesterol efflux protein ABCA1
by: Shujiang Zhuo, et al.
Published: (2024-05-01)

ApoA-I deficiency causes both hypertriglyceridemia and increased atherosclerosis in human apoB transgenic mice
by: Emanuel Voyiaziakis, et al.
Published: (1998-02-01)

Rat McA-RH7777 cells efficiently assemble rat apolipoprotein B-48 or larger fragments into VLDL but not human apolipoprotein B of any size
by: Qunong Xiao, et al.
Published: (2000-01-01)

Effects of estrogenic oral contraceptives on the lipoprotein B particle system defined by apolipoproteins E and C-III content
by: Christina Khoo, et al.
Published: (1999-02-01)

Erythrodiol, an Olive Oil Constituent, Increases the Half-Life of ABCA1 and Enhances Cholesterol Efflux from THP-1-Derived Macrophages
by: Limei Wang, et al.
Published: (2017-06-01)

The Dietary Constituent Falcarindiol Promotes Cholesterol Efflux from THP-1 Macrophages by Increasing ABCA1 Gene Transcription and Protein Stability
by: Limei Wang, et al.
Published: (2017-09-01)

Spectroscopic and In Vitro Investigations of Boron(III) Complex with <i>Meso</i>-4-Methoxycarbonylpropylsubstituted Dipyrromethene for Fluorescence Bioimaging Applications
by: Galina Guseva, et al.
Published: (2020-10-01)

Genotypic associations of the hepatic secretion of VLDL apolipoprotein B-100 in obesity
by: G.F. Watts, et al.
Published: (2000-03-01)

Enhanced atherosclerosis in apolipoprotein E knockout rabbits: role of apoB48-rich remnant lipoproteins
by: Manabu Niimi, et al.
Published: (2024-07-01)

Mechanisms modulating foam cell formation in the arterial intima: exploring new therapeutic opportunities in atherosclerosis
by: M. T. La Chica Lhoëst, et al.
Published: (2024-06-01)

Apolipoprotein B and non-high-density lipoprotein cholesterol reveal a high atherogenicity in individuals with type 2 diabetes and controlled low-density lipoprotein-cholesterol
by: Liliana Fonseca, et al.
Published: (2020-06-01)

Ezetimibe in Combination With Simvastatin Reduces Remnant Cholesterol Without Affecting Biliary Lipid Concentrations in Gallstone Patients
by: Osman Ahmed, et al.
Published: (2018-12-01)

Lower plasma PCSK9 in normocholesterolemic subjects is associated with upregulated adipose tissue surface‐expression of LDLR and CD36 and NLRP3 inflammasome
by: Yannick Cyr, et al.
Published: (2021-02-01)

Role of high-density lipoprotein cholesterol in health and diseases
by: Brijesh Yadav, et al.
Published: (2021-01-01)

Cell cholesterol efflux: integration of old and new observations provides new insights
by: George H. Rothblat, et al.
Published: (1999-05-01)

Macrophage Cholesterol Efflux Downregulation Is Not Associated with Abdominal Aortic Aneurysm (AAA) Progression
by: Marina Canyelles, et al.
Published: (2020-04-01)

The roles of different pathways in the release of cholesterol from macrophages
by: Maria Pia Adorni, et al.
Published: (2007-11-01)

Association of cerebrospinal fluid NPY with peripheral ApoA: a moderation effect of BMI
by: Danyang Zhao, et al.
Published: (2024-07-01)

ASSOCIATION OF CAROTID INTIMA-MEDIA THICKNESS WITH LEPTIN AND APOLIPOPROTEIN B/APOLIPOPROTEIN A-I RATIO REVEALS IMMINENT PREDICTORS OF SUBCLINICAL ATHEROSCLEROSIS IN PSORIASIS PATIENTS
by: Kumari Asha, et al.
Published: (2014-01-01)

Role of apoA-II in lipid metabolism and atherosclerosis: advances in the study of an enigmatic protein
by: Francisco Blanco-Vaca, et al.
Published: (2001-11-01)