Efficient knockin mouse generation by ssDNA oligonucleotides and zinc-finger nuclease assisted homologous recombination in zygotes.

Efficient knockin mouse generation by ssDNA oligonucleotides and zinc-finger nuclease assisted homologous recombination in zygotes.

The generation of specific mutant animal models is critical for functional analysis of human genes. The conventional gene targeting approach in embryonic stem cells (ESCs) by homologous recombination is however laborious, slow, expensive, and limited to species with functional ESCs. It is therefore...

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Bibliographic Details
Main Authors: Bin Shen, Xin Zhang, Yinan Du, Jianying Wang, Jun Gong, Xiaodong Zhang, Peri H Tate, Hongliang Li, Xingxu Huang, Wensheng Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3805579?pdf=render

Internet

http://europepmc.org/articles/PMC3805579?pdf=render

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