Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice.
Peripheral CD8(+) T cells are defective in both IL-15 and IL-15Rα knock-out (KO) mice; however, whether IL-15/IL-15Rα deficiency has a similar effect on CD8 single-positive (SP) thymocytes remains unclear. In this study, we investigated whether the absence of IL-15 transpresentation in IL-15Rα KO mi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3308975?pdf=render |
| _version_ | 1818564866898657280 |
|---|---|
| author | Kai-Ping N Chow Jian-Tai Qiu Jam-Mou Lee Shuo-Lun Hsu Shan-Che Yang Ning-Ning Wu Wei Huang Tzong-Shoon Wu |
| author_facet | Kai-Ping N Chow Jian-Tai Qiu Jam-Mou Lee Shuo-Lun Hsu Shan-Che Yang Ning-Ning Wu Wei Huang Tzong-Shoon Wu |
| author_sort | Kai-Ping N Chow |
| collection | DOAJ |
| description | Peripheral CD8(+) T cells are defective in both IL-15 and IL-15Rα knock-out (KO) mice; however, whether IL-15/IL-15Rα deficiency has a similar effect on CD8 single-positive (SP) thymocytes remains unclear. In this study, we investigated whether the absence of IL-15 transpresentation in IL-15Rα KO mice results in a defect in thymic CD8 single positive (SP) TCR(hi) thymocytes. Comparison of CD8SP TCR(hi) thymocytes from IL-15Rα KO mice with their wild type (WT) counterparts by flow cytometry showed a significant reduction in the percentage of CD69(-) CD8SP TCR(hi) thymocytes, which represent thymic premigrants. In addition, analysis of in vivo 5-bromo-2-deoxyuridine (BrdU) incorporation demonstrated that premigrant expansion of CD8SP TCR(hi) thymocytes was reduced in IL-15Rα KO mice. The presence of IL-15 transpresentation-dependent expansion in CD8SP TCR(hi) thymocytes was assessed by culturing total thymocytes in IL-15Rα-Fc fusion protein-pre-bound plates that were pre-incubated with IL-15 to mimic IL-15 transpresentation in vitro. The results demonstrated that CD8SP thymocytes selectively outgrew other thymic subsets. The contribution of the newly divided CD8SP thymocytes to the peripheral CD8(+) T cell pool was examined using double labeling with intrathymically injected FITC and intravenously injected BrdU. A marked decrease in FITC(+) BrdU(+) CD8(+) T cells was observed in the IL-15Rα KO lymph nodes. Through these experiments, we identified an IL-15 transpresentation-dependent proliferation process selective for the mature CD8SP premigrant subpopulation. Importantly, this process may contribute to the maintenance of the normal peripheral CD8(+) T cell pool. |
| first_indexed | 2024-12-14T01:34:07Z |
| format | Article |
| id | doaj.art-d100bc280cb24ac0a79eae0b9ba11e76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T01:34:07Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d100bc280cb24ac0a79eae0b9ba11e762022-12-21T23:21:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3315210.1371/journal.pone.0033152Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice.Kai-Ping N ChowJian-Tai QiuJam-Mou LeeShuo-Lun HsuShan-Che YangNing-Ning WuWei HuangTzong-Shoon WuPeripheral CD8(+) T cells are defective in both IL-15 and IL-15Rα knock-out (KO) mice; however, whether IL-15/IL-15Rα deficiency has a similar effect on CD8 single-positive (SP) thymocytes remains unclear. In this study, we investigated whether the absence of IL-15 transpresentation in IL-15Rα KO mice results in a defect in thymic CD8 single positive (SP) TCR(hi) thymocytes. Comparison of CD8SP TCR(hi) thymocytes from IL-15Rα KO mice with their wild type (WT) counterparts by flow cytometry showed a significant reduction in the percentage of CD69(-) CD8SP TCR(hi) thymocytes, which represent thymic premigrants. In addition, analysis of in vivo 5-bromo-2-deoxyuridine (BrdU) incorporation demonstrated that premigrant expansion of CD8SP TCR(hi) thymocytes was reduced in IL-15Rα KO mice. The presence of IL-15 transpresentation-dependent expansion in CD8SP TCR(hi) thymocytes was assessed by culturing total thymocytes in IL-15Rα-Fc fusion protein-pre-bound plates that were pre-incubated with IL-15 to mimic IL-15 transpresentation in vitro. The results demonstrated that CD8SP thymocytes selectively outgrew other thymic subsets. The contribution of the newly divided CD8SP thymocytes to the peripheral CD8(+) T cell pool was examined using double labeling with intrathymically injected FITC and intravenously injected BrdU. A marked decrease in FITC(+) BrdU(+) CD8(+) T cells was observed in the IL-15Rα KO lymph nodes. Through these experiments, we identified an IL-15 transpresentation-dependent proliferation process selective for the mature CD8SP premigrant subpopulation. Importantly, this process may contribute to the maintenance of the normal peripheral CD8(+) T cell pool.http://europepmc.org/articles/PMC3308975?pdf=render |
| spellingShingle | Kai-Ping N Chow Jian-Tai Qiu Jam-Mou Lee Shuo-Lun Hsu Shan-Che Yang Ning-Ning Wu Wei Huang Tzong-Shoon Wu Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. PLoS ONE |
| title | Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. |
| title_full | Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. |
| title_fullStr | Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. |
| title_full_unstemmed | Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. |
| title_short | Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. |
| title_sort | selective reduction of post selection cd8 thymocyte proliferation in il 15rα deficient mice |
| url | http://europepmc.org/articles/PMC3308975?pdf=render |
| work_keys_str_mv | AT kaipingnchow selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT jiantaiqiu selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT jammoulee selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT shuolunhsu selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT shancheyang selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT ningningwu selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT weihuang selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice AT tzongshoonwu selectivereductionofpostselectioncd8thymocyteproliferationinil15radeficientmice |