Comparative Analysis of Universal Protein Extraction Methodologies for Screening of Lipase Activity from Agricultural Products

Comparative Analysis of Universal Protein Extraction Methodologies for Screening of Lipase Activity from Agricultural Products

Protein extraction techniques are absolutely required for the research of biological catalysts. The present study compared four universal protein extraction methodologies (ammonium sulfate precipitation, TCA/acetone precipitation, and two commercial kits) to provide practical information on protein...

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Bibliographic Details
Main Authors: Jisu Ha, Jun-Young Park, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Catalysts
Subjects:
lipase
agricultural product
protein extraction
extraction yield
specific activity
spectrophotometric assay
Online Access:https://www.mdpi.com/2073-4344/11/7/816

Internet

https://www.mdpi.com/2073-4344/11/7/816

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