Overlooked tools for studying soil nitrogen depolymerization: Aminopeptidase assays using nitroanilide substrates

Abstract Aminopeptidases are one of the extracellular hydrolytic enzymes that catalyze organic nitrogen (N) depolymerization and are commonly assayed using fluorogenic substrates. However, chromogenic substrates based on para‐nitroaniline (pNA) developed for the study of aminopeptidases in the 1960s...

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Bibliographic Details
Main Authors: Andrew J Margenot, Rachel C Daughtridge
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Agricultural & Environmental Letters
Online Access:https://doi.org/10.1002/ael2.20079