Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat
Abstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45...
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Nature Portfolio
2017-05-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-01769-2 |
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author | Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu |
author_facet | Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu |
author_sort | Tsong-Hai Lee |
collection | DOAJ |
description | Abstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45 mg/kg rt-PA, (B) intravenous bolus injection of 0.9 mg/kg, (C) sonothrombolysis with FUS alone, (D) oral administration of 2 g/kg BNG-1 for 7 days alone, (E) A + D, (F) A + C, and (G) A + C + D. Four comparison groups were made including (H) 0.45 mg/kg rt-PA 20% bolus +80% IV fusion + FUS, (I) 0.9 mg/kg rt-PA with 10% bolus + 90% intravenous fusion, (J) B + C, (K) B + D. At 7 days after carotid occlusion, small-animal carotid ultrasound and 7 T MR angiography showed the recanalization rate of ≤50% stenosis was 50% in group B and 83% in group I, but 0% in groups A and C and 17% in group D. Combination therapy improved recanalization rate to 50–63% in groups E and F, to 67–83% in groups J and K, and to 100% in groups G and H. Our study demonstrated combination therapy with different remedies can be a feasible strategy to improve the thrombolytic effect of rt-PA. |
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spelling | doaj.art-d12ec1f03e28435994faa940938b6daa2022-12-21T20:35:51ZengNature PortfolioScientific Reports2045-23222017-05-017111110.1038/s41598-017-01769-2Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in ratTsong-Hai Lee0Jih-Chao Yeh1Chih-Hung Tsai2Jen-Tsung Yang3Shyh-Liang Lou4Chen-June Seak5Chao-Yung Wang6Kuo-Chen Wei7Hao-Li Liu8Stroke Center and Department of Neurology, Linkou Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityStroke Center and Department of Neurology, Linkou Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityDepartments of Electrical Engineering, Graduate Institute of Clinical Medical Sciences, Chang-Gung UniversityDepartment of Neurosurgery, Chiayi Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityDepartment of Biomedical Engineering, Chung Yuan Christian UniversityDepartment of Emergency Medicine, Linkou Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityDepartment of Cardiology, Linkou Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityDepartment of Neurosurgery, Linkou Chang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityDepartments of Electrical Engineering, Graduate Institute of Clinical Medical Sciences, Chang-Gung UniversityAbstract Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45 mg/kg rt-PA, (B) intravenous bolus injection of 0.9 mg/kg, (C) sonothrombolysis with FUS alone, (D) oral administration of 2 g/kg BNG-1 for 7 days alone, (E) A + D, (F) A + C, and (G) A + C + D. Four comparison groups were made including (H) 0.45 mg/kg rt-PA 20% bolus +80% IV fusion + FUS, (I) 0.9 mg/kg rt-PA with 10% bolus + 90% intravenous fusion, (J) B + C, (K) B + D. At 7 days after carotid occlusion, small-animal carotid ultrasound and 7 T MR angiography showed the recanalization rate of ≤50% stenosis was 50% in group B and 83% in group I, but 0% in groups A and C and 17% in group D. Combination therapy improved recanalization rate to 50–63% in groups E and F, to 67–83% in groups J and K, and to 100% in groups G and H. Our study demonstrated combination therapy with different remedies can be a feasible strategy to improve the thrombolytic effect of rt-PA.https://doi.org/10.1038/s41598-017-01769-2 |
spellingShingle | Tsong-Hai Lee Jih-Chao Yeh Chih-Hung Tsai Jen-Tsung Yang Shyh-Liang Lou Chen-June Seak Chao-Yung Wang Kuo-Chen Wei Hao-Li Liu Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat Scientific Reports |
title | Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
title_full | Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
title_fullStr | Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
title_full_unstemmed | Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
title_short | Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
title_sort | improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat |
url | https://doi.org/10.1038/s41598-017-01769-2 |
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