Effect of very long-term storage and multiple freeze and thaw cycles on 11-dehydro-thromboxane-B2 and 8-iso-prostaglandin F2α, levels in human urine samples by validated enzyme immunoassays

Abstract Biological samples are often frozen and stored for years and/or thawed multiple times, thus assessing their stability on long-term storage and repeated freeze–thaw cycles is crucial. The study aims were to assess:—the long-term stability of two major enzymatic and non-enzymatic metabolites...

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Bibliographic Details
Main Authors: Giovanna Petrucci, Duaa Hatem, Ruth Langley, Siobhan Cleary, Aleksandra Gentry-Maharaj, Dario Pitocco, Alessandro Rizzi, Paola Ranalli, Francesco Zaccardi, Aida Habib, Bianca Rocca
Format: Article
Language:English
Published: Nature Portfolio 2024-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-55720-3