Cell cycle specific, differentially tagged ribosomal proteins to measure phase specific transcriptomes from asynchronously cycling cells

Abstract Asynchronously cycling cells pose a challenge to the accurate characterization of phase-specific gene expression. Current strategies, including RNAseq, survey the steady state gene expression across the cell cycle and are inherently limited by their inability to resolve dynamic gene regulat...

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Bibliographic Details
Main Authors: Jesse D. Cochran, Tess A. Leathers, Emir Maldosevic, Klara W. Siejda, Julian Vitello, Haesol Lee, Leigh A. Bradley, Alex Young, Ahmad Jomaa, Matthew J. Wolf
Format: Article
Language:English
Published: Nature Portfolio 2024-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-52085-5