Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853

Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853

Abstract Kanamycin B as the secondary metabolite of wild‐type Streptomyces kanamyceticus (S. kanamyceticus) ATCC12853 is often used for the synthesis of dibekacin and arbekacin. To construct the strain has the ability for kanamycin B production; the pSET152 derivatives from Escherichia coli ET12567...

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Bibliographic Details
Main Authors: Shuman Zhang, Tiansheng Chen, Jia Jia, Liwen Guo, Huizheng Zhang, Chao Li, Renzhong Qiao
Format: Article
Language:English
Published: Wiley 2019-06-01
Series:MicrobiologyOpen
Subjects:
conjugal transfer
gene knockout
kanamycin
kanamycin B
Streptomyces kanamyceticus
Online Access:https://doi.org/10.1002/mbo3.747

Internet

https://doi.org/10.1002/mbo3.747

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