Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans

Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans

ABSTRACTMecA is a highly conserved adaptor protein encoded by prokaryotes from the Bacillota phylum. MecA mutants exhibit similar pleiotropic defects in a variety of organisms, although most of these phenotypes currently lack a mechanistic basis. MecA mediates ClpCP-dependent proteolysis of its subs...

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Bibliographic Details
Main Authors: Hua Qin, David Anderson, Zhengzhong Zou, Dustin Higashi, Christina Borland, Jens Kreth, Justin Merritt
Format: Article
Language:English
Published: American Society for Microbiology 2024-02-01
Series:Microbiology Spectrum
Subjects:
Streptococcus mutans
MecA interactome
split luciferase complementation assays
mass spectrometry
protein binding affinity
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03691-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.03691-23

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