First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth
Background: The interplay between vitamin K (vitK) (as carboxylation cofactor, partially produced by the gut microbiota) and short-chain fatty acids (SCFAs), the end-product of fiber fermentation in the gut, has never been assessed in mother–newborn pairs, although newborns are considered vitK defic...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/3/847 |
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| author | Tamás Ilyés Marius Pop Mihai Surcel Daria M. Pop Răzvan Rusu Ciprian N. Silaghi Gabriela C. Zaharie Alexandra M. Crăciun |
| author_facet | Tamás Ilyés Marius Pop Mihai Surcel Daria M. Pop Răzvan Rusu Ciprian N. Silaghi Gabriela C. Zaharie Alexandra M. Crăciun |
| author_sort | Tamás Ilyés |
| collection | DOAJ |
| description | Background: The interplay between vitamin K (vitK) (as carboxylation cofactor, partially produced by the gut microbiota) and short-chain fatty acids (SCFAs), the end-product of fiber fermentation in the gut, has never been assessed in mother–newborn pairs, although newborns are considered vitK deficient and with sterile gut. Methods: We collected venous blood from 45 healthy mothers with uncomplicated term pregnancies and umbilical cord blood from their newborns at birth. The concentrations of total SCFAs and hepatic/extra-hepatic vitK-dependent proteins (VKDPs), as proxies of vitK status were assayed: undercarboxylated and total matrix Gla protein (ucMGP and tMGP), undercarboxylated osteocalcin (ucOC), undercarboxylated Gla-rich protein (ucGRP), and protein induced by vitK absence II (PIVKA-II). Results: We found significantly higher ucOC (18.6-fold), ucMGP (9.2-fold), and PIVKA-II (5.6-fold) levels in newborns, while tMGP (5.1-fold) and SCFAs (2.4-fold) were higher in mothers, and ucGRP was insignificantly modified. In mother–newborn pairs, only ucGRP (r = 0.746, <i>p</i> < 0.01) and SCFAs (r = 0.428, <i>p</i> = 0.01) levels were correlated. <b>Conclusions:</b> We report for the first time the presence of SCFAs in humans at birth, probably transferred through the placenta to the fetus. The increased circulating undercarboxylated VKDPSs in newborns revealed a higher vitamin K deficiency at the extrahepatic level compared to liver VKDPs. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:40Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-d187112b8e53403e864a311464d5e3472023-11-17T12:13:11ZengMDPI AGLife2075-17292023-03-0113384710.3390/life13030847First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at BirthTamás Ilyés0Marius Pop1Mihai Surcel2Daria M. Pop3Răzvan Rusu4Ciprian N. Silaghi5Gabriela C. Zaharie6Alexandra M. Crăciun7Department of Molecular Sciences, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaDepartment of Molecular Sciences, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, Romania1st Obstetrics and Gynecology Department, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, Romania2nd Obstetrics and Gynecology Department, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaDepartment of Molecular Sciences, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaDepartment of Molecular Sciences, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaDepartment of Neonatology, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaDepartment of Molecular Sciences, University of Medicine and Pharmacy “Iuliu Hațieganu”, 400012 Cluj-Napoca, RomaniaBackground: The interplay between vitamin K (vitK) (as carboxylation cofactor, partially produced by the gut microbiota) and short-chain fatty acids (SCFAs), the end-product of fiber fermentation in the gut, has never been assessed in mother–newborn pairs, although newborns are considered vitK deficient and with sterile gut. Methods: We collected venous blood from 45 healthy mothers with uncomplicated term pregnancies and umbilical cord blood from their newborns at birth. The concentrations of total SCFAs and hepatic/extra-hepatic vitK-dependent proteins (VKDPs), as proxies of vitK status were assayed: undercarboxylated and total matrix Gla protein (ucMGP and tMGP), undercarboxylated osteocalcin (ucOC), undercarboxylated Gla-rich protein (ucGRP), and protein induced by vitK absence II (PIVKA-II). Results: We found significantly higher ucOC (18.6-fold), ucMGP (9.2-fold), and PIVKA-II (5.6-fold) levels in newborns, while tMGP (5.1-fold) and SCFAs (2.4-fold) were higher in mothers, and ucGRP was insignificantly modified. In mother–newborn pairs, only ucGRP (r = 0.746, <i>p</i> < 0.01) and SCFAs (r = 0.428, <i>p</i> = 0.01) levels were correlated. <b>Conclusions:</b> We report for the first time the presence of SCFAs in humans at birth, probably transferred through the placenta to the fetus. The increased circulating undercarboxylated VKDPSs in newborns revealed a higher vitamin K deficiency at the extrahepatic level compared to liver VKDPs.https://www.mdpi.com/2075-1729/13/3/847short-chain fatty acidsvitamin-K-dependent proteinsmatrix Gla proteinosteocalcinGla-rich proteinPIVKA |
| spellingShingle | Tamás Ilyés Marius Pop Mihai Surcel Daria M. Pop Răzvan Rusu Ciprian N. Silaghi Gabriela C. Zaharie Alexandra M. Crăciun First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth Life short-chain fatty acids vitamin-K-dependent proteins matrix Gla protein osteocalcin Gla-rich protein PIVKA |
| title | First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth |
| title_full | First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth |
| title_fullStr | First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth |
| title_full_unstemmed | First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth |
| title_short | First Comparative Evaluation of Short-Chain Fatty Acids and Vitamin-K-Dependent Proteins Levels in Mother–Newborn Pairs at Birth |
| title_sort | first comparative evaluation of short chain fatty acids and vitamin k dependent proteins levels in mother newborn pairs at birth |
| topic | short-chain fatty acids vitamin-K-dependent proteins matrix Gla protein osteocalcin Gla-rich protein PIVKA |
| url | https://www.mdpi.com/2075-1729/13/3/847 |
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