Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

Abstract Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers. However, transcriptome analysis remains challenging with standard mRNA-seq methods as FFPE derived-RNA...

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Bibliographic Details
Main Authors: Jin Sung Jang, Eileen Holicky, Julie Lau, Samantha McDonough, Mark Mutawe, Matthew J. Koster, Kenneth J. Warrington, Julie M. Cuninngham
Format: Article
Language:English
Published: BMC 2021-10-01
Series:BMC Genomics
Subjects:
3′ mRNA-Seq
UMI
FFPE
PCR amplification bias
Gene expression
Online Access:https://doi.org/10.1186/s12864-021-08068-1

Internet

https://doi.org/10.1186/s12864-021-08068-1

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