Targeted integration of EpCAM-specific CAR in human induced pluripotent stem cells and their differentiation into NK cells

Targeted integration of EpCAM-specific CAR in human induced pluripotent stem cells and their differentiation into NK cells

Abstract Background Redirection of natural killer (NK) cells with chimeric antigen receptors (CAR) is attractive in developing off-the-shelf CAR therapeutics for cancer treatment. However, the site-specific integration of a CAR gene into NK cells remains challenging. Methods In the present study, we...

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Bibliographic Details
Main Authors: Shin Yi Tang, Shijun Zha, Zhicheng Du, Jieming Zeng, Detu Zhu, Yumei Luo, Shu Wang
Format: Article
Language:English
Published: BMC 2021-11-01
Series:Stem Cell Research & Therapy
Subjects:
Induced pluripotent stem cells (iPSC)
Natural killer cells (NK)
Chimeric antigen receptors (CAR)
Adeno-associated virus integration site 1 (AAVS1)
Zinc finger nuclease (ZFN)
Genetic engineering
Online Access:https://doi.org/10.1186/s13287-021-02648-4

Internet

https://doi.org/10.1186/s13287-021-02648-4

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