Despite low viral titer in saliva samples, Sanger-based SARS-CoV-2 spike gene sequencing is highly applicable for the variant identification

Despite low viral titer in saliva samples, Sanger-based SARS-CoV-2 spike gene sequencing is highly applicable for the variant identification

Abstract Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241 stocked saliva samples collected fro...

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Bibliographic Details
Main Authors: Ko Ko, Kazuaki Takahashi, Noriaki Ito, Aya Sugiyama, Shintaro Nagashima, Kei Miwata, Yoshihiro Kitahara, Mafumi Okimoto, Serge Ouoba, Golda Ataa Akuffo, Bunthen E, Tomoyuki Akita, Toshiro Takafuta, Junko Tanaka
Format: Article
Language:English
Published: BMC 2023-08-01
Series:BMC Medical Genomics
Subjects:
SARS-CoV-2
Sanger method
Next generation sequencing
Amplification
Variants
Screening
Online Access:https://doi.org/10.1186/s12920-023-01633-5

Internet

https://doi.org/10.1186/s12920-023-01633-5

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