Optimized Recombinant Expression and Characterization of Collagenase in <i>Bacillus subtilis</i> WB600

Optimized Recombinant Expression and Characterization of Collagenase in <i>Bacillus subtilis</i> WB600

Background: The collagenase encoding gene <i>col</i> was cloned into a pP43NMK vector and amplified in <i>Escherichia coli</i> JM109 cells. The shuttle vector pP43NMK was used to sub-clone the <i>col</i> gene to obtain the vector pP43NMK-<i>col</i> for...

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Bibliographic Details
Main Authors: Yaqing Zhu, Linlin Wang, Kaixuan Zheng, Ping Liu, Wenkang Li, Jian Lin, Wenjing Liu, Shoushui Shan, Liqin Sun, Hailing Zhang
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Fermentation
Subjects:
collagenase
<i>Bacillus subtilis</i> WB600
protein purification
characterization
recombinant protein expression
Online Access:https://www.mdpi.com/2311-5637/8/9/449

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https://www.mdpi.com/2311-5637/8/9/449

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