Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Summary: Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinica...

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Bibliographic Details
Main Authors: Soichiro Sonoda, Haruyoshi Yamaza, Koichiro Yoshimaru, Tomoaki Taguchi, Takayoshi Yamaza
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002660