Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
Summary: Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinica...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002660 |