Heritable and inducible gene knockdown in astrocytes or neurons in vivo by a combined lentiviral and RNAi approach.

Heritable and inducible gene knockdown in astrocytes or neurons in vivo by a combined lentiviral and RNAi approach.

Gene knockout by homologous recombination is a popular method to study gene functions in the mouse in vivo. However, its lack of temporal control has limited the interpretation of knockout studies because the complete elimination of a gene product often alters developmental processes, and can induce...

Full description

Bibliographic Details
Main Authors: Fabrice eHeitz, Torbjörn eJohansson, Karsten eBaumgärtel, Rreze eGecaj, Pawel ePelczar, Isabelle M Mansuy
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-03-01
Series:Frontiers in Cellular Neuroscience
Subjects:
Cre-lox genetics
shRNA
In vivo gene knockdown
conditional gene knockdown
lentivirus-mediated transgenesis
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00062/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00062/full

Similar Items