Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach
The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimi...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4241 |
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| author | Jesús Alfredo Araujo-León Rolffy Ortiz-Andrade Rivelino Armando Vera-Sánchez Julio Enrique Oney-Montalvo Tania Isolina Coral-Martínez Zulema Cantillo-Ciau |
| author_facet | Jesús Alfredo Araujo-León Rolffy Ortiz-Andrade Rivelino Armando Vera-Sánchez Julio Enrique Oney-Montalvo Tania Isolina Coral-Martínez Zulema Cantillo-Ciau |
| author_sort | Jesús Alfredo Araujo-León |
| collection | DOAJ |
| description | The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (<i>p</i> > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (<i>p</i> < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids. |
| first_indexed | 2024-03-10T16:18:59Z |
| format | Article |
| id | doaj.art-d25ed0f0c1d64621bc06c2e9e2273dff |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:18:59Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d25ed0f0c1d64621bc06c2e9e2273dff2023-11-20T13:51:49ZengMDPI AGMolecules1420-30492020-09-012518424110.3390/molecules25184241Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic ApproachJesús Alfredo Araujo-León0Rolffy Ortiz-Andrade1Rivelino Armando Vera-Sánchez2Julio Enrique Oney-Montalvo3Tania Isolina Coral-Martínez4Zulema Cantillo-Ciau5Chromatography Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoPharmacology Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoChromatography Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoChromatography Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoChromatography Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoMedicinal Chemistry Laboratory, Chemistry Faculty, Autonomous University of Yucatan, Mérida 97069, MexicoThe purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (<i>p</i> > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (<i>p</i> < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.https://www.mdpi.com/1420-3049/25/18/4241ESIflavonoidoptimizationpharmacokineticvalidationQqQ |
| spellingShingle | Jesús Alfredo Araujo-León Rolffy Ortiz-Andrade Rivelino Armando Vera-Sánchez Julio Enrique Oney-Montalvo Tania Isolina Coral-Martínez Zulema Cantillo-Ciau Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach Molecules ESI flavonoid optimization pharmacokinetic validation QqQ |
| title | Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach |
| title_full | Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach |
| title_fullStr | Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach |
| title_full_unstemmed | Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach |
| title_short | Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach |
| title_sort | development and optimization of a high sensitivity lc ms ms method for the determination of hesperidin and naringenin in rat plasma pharmacokinetic approach |
| topic | ESI flavonoid optimization pharmacokinetic validation QqQ |
| url | https://www.mdpi.com/1420-3049/25/18/4241 |
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