Propidium Monoazide Combined With RT-qPCR Detects Infectivity of Porcine Epidemic Diarrhea Virus

Propidium Monoazide Combined With RT-qPCR Detects Infectivity of Porcine Epidemic Diarrhea Virus

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) allows sensitive detection of viral particles and viruses in epidemic samples but it cannot discriminate noninfectious viruses from infectious ones. Propidium monoazide (PMA) coupled with quantitative polymerase chain reaction (q...

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Bibliographic Details
Main Authors: Gong Liang, Yunzhi Long, Qianqian Li, Liu Yang, Ying Huang, Daobing Yu, Wenbo Song, Mingguang Zhou, Gaoyuan Xu, Chao Huang, Xibiao Tang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-07-01
Series:Frontiers in Veterinary Science
Subjects:
RT-qPCR
PEDV
PMA
infectivity
examine
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2022.931392/full

Internet

https://www.frontiersin.org/articles/10.3389/fvets.2022.931392/full

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