First thermostable CLIP-tag by rational design applied to an archaeal O6-alkyl-guanine-DNA-alkyl-transferase

First thermostable CLIP-tag by rational design applied to an archaeal O6-alkyl-guanine-DNA-alkyl-transferase

Self-labelling protein tags (SLPs) are resourceful tools that revolutionized sensor imaging, having the versatile ability of being genetically fused with any protein of interest and undergoing activation with alternative probes specifically designed for each variant (namely, SNAP-tag, CLIP-tag and H...

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Bibliographic Details
Main Authors: Rosa Merlo, Rosanna Mattossovich, Marianna Genta, Anna Valenti, Giovanni Di Mauro, Alberto Minassi, Riccardo Miggiano, Giuseppe Perugino
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
Protein-tag
Protein labelling
Orthogonal substrate specificity
Thermozymes
Protein engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037022004226

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http://www.sciencedirect.com/science/article/pii/S2001037022004226

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