Cryopreservation by vitritication of carrot (Daucus carota L.) suspension cells and protoplasts(胡萝卜悬浮培养细胞和原生质体的玻璃化法超低温保存)

采用玻璃化法成功地超低温保存了胡萝卜悬浮培养细胞及其原生质体,存活率分别为83.3%(TTC法检测)和47%(FDA法检测).冻后细胞能恢复生长并再生植株.分析了玻璃化法超低温保存植物悬培养浮细胞和原生质体的主要影响因素.

Bibliographic Details
Main Authors:	CHENYong(陈勇), WANGJun-hui(王君晖), HUANGChun-nong(黄纯农)
Format:	Article
Language:	zho
Published:	Zhejiang University Press 2002-01-01
Series:	Zhejiang Daxue xuebao. Lixue ban
Subjects:	玻璃化法超低温保存胡萝卜悬浮细胞原生质体植株再生
Online Access:	https://doi.org/zjup/1008-9497.2002.29.1.94-98

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