Protocol for detecting lncRNA-protein interaction in vivo using the yeast three-hybrid assay

Protocol for detecting lncRNA-protein interaction in vivo using the yeast three-hybrid assay

Summary: Analyses of long non-coding RNA (lncRNA)-protein interactions provide key clues for understanding the molecular basis of lncRNA-modulated biological processes. Here, we detail a yeast three-hybrid assay to identify the lncRNA-interacting protein. We describe steps for lncRNA bait preparatio...

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Bibliographic Details
Main Authors: Jingjing Cai, Reqing He, Yongdi Zhang, Pengxiang Zhang, Youlin Zhu, Dong Wang
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Genetics
Plant sciences
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000212

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724000212

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