A mimicking-of-DNA-methylation-patterns pipeline for overcoming the restriction barrier of bacteria.
Genetic transformation of bacteria harboring multiple Restriction-Modification (R-M) systems is often difficult using conventional methods. Here, we describe a mimicking-of-DNA-methylation-patterns (MoDMP) pipeline to address this problem in three difficult-to-transform bacterial strains. Twenty-fou...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-09-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3459991?pdf=render |