Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting

Summary: BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting....

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Bibliographic Details
Main Authors: Yuzuru Sasamoto, Philip C. Yeung, Johnathan Tran, Markus H. Frank, Natasha Y. Frank
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004707