Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning
This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is high...
| Main Authors: | , , , , , , , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
Bio-protocol LLC
2017-11-01
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| 叢編: | Bio-Protocol |
| 在線閱讀: | https://bio-protocol.org/en/bpdetail?id=2615&type=0 |
| 總結: | This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from any synthesizing company. The protocol drastically reduces time and handling between receiving the synthesized DNA fragments and transforming with vector and DNA fragment(s). |
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| ISSN: | 2331-8325 |