<i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products
The food industry is constantly struggling with one of the most prevalent biofilm-forming and food-borne pathogenic bacteria, <i>Campylobacter jejuni</i>. Different approaches are used to control biofilms in the food production chain, but none is fully effective. In this study, we aim to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/7/854 |
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| author | Dina Ramić Janja Ogrizek Franz Bucar Barbka Jeršek Miha Jeršek Sonja Smole Možina |
| author_facet | Dina Ramić Janja Ogrizek Franz Bucar Barbka Jeršek Miha Jeršek Sonja Smole Možina |
| author_sort | Dina Ramić |
| collection | DOAJ |
| description | The food industry is constantly struggling with one of the most prevalent biofilm-forming and food-borne pathogenic bacteria, <i>Campylobacter jejuni</i>. Different approaches are used to control biofilms in the food production chain, but none is fully effective. In this study, we aim to produce and determine the chemical profile of essential oils (EOs), ethanolic extracts of flowers prior to distillation (EFs), and ethanolic extracts of post-distillation waste material (EWMs) from <i>Lavandula × intermedia</i> ‘Bila’, ‘Budrovka’ St Nicholas and ‘Budrovka’, which were further used to reduce <i>C. jejuni</i> intercellular signaling, adhesion, and biofilm formation, as well as to test their antioxidant activity. Glycosides of hydroxycinnamic acids were the major constituents of both types of lavandin ethanolic extract, while linalool, linalyl acetate, 1,8-cineol, and camphor were the major compounds found in lavandin EOs. Tested EOs showed the best antibacterial activity with a minimal inhibitory concentration of 0.25 mg/mL. Lavandin EFs proved more effective in reducing <i>C. jejuni</i> intercellular signaling and adhesion compared to lavandin EOs and EWMs, while lavandin EOs showed a slightly better effect against biofilm formation. Interestingly, the best antioxidant activity was determined for lavandin EWMs. A positive and moderate correlation was found between the reduction of <i>C. jejuni</i> intercellular signaling and adhesion, as well as between adhesion and biofilm formation. These findings mean novel bacterial targets are of interest for biofilm control with alternative natural agents throughout the whole food production chain. |
| first_indexed | 2024-03-09T03:47:50Z |
| format | Article |
| id | doaj.art-d2ade96384e54a049c5183958fbe42fb |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T03:47:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d2ade96384e54a049c5183958fbe42fb2023-12-03T14:32:29ZengMDPI AGAntibiotics2079-63822022-06-0111785410.3390/antibiotics11070854<i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-ProductsDina Ramić0Janja Ogrizek1Franz Bucar2Barbka Jeršek3Miha Jeršek4Sonja Smole Možina5Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaDepartment of Pharmacognosy, Institute for Pharmaceutical Sciences, University of Graz, A-8010 Graz, AustriaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaSlovenian Museum of Natural History, Prešernova Cesta 20, 1001 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThe food industry is constantly struggling with one of the most prevalent biofilm-forming and food-borne pathogenic bacteria, <i>Campylobacter jejuni</i>. Different approaches are used to control biofilms in the food production chain, but none is fully effective. In this study, we aim to produce and determine the chemical profile of essential oils (EOs), ethanolic extracts of flowers prior to distillation (EFs), and ethanolic extracts of post-distillation waste material (EWMs) from <i>Lavandula × intermedia</i> ‘Bila’, ‘Budrovka’ St Nicholas and ‘Budrovka’, which were further used to reduce <i>C. jejuni</i> intercellular signaling, adhesion, and biofilm formation, as well as to test their antioxidant activity. Glycosides of hydroxycinnamic acids were the major constituents of both types of lavandin ethanolic extract, while linalool, linalyl acetate, 1,8-cineol, and camphor were the major compounds found in lavandin EOs. Tested EOs showed the best antibacterial activity with a minimal inhibitory concentration of 0.25 mg/mL. Lavandin EFs proved more effective in reducing <i>C. jejuni</i> intercellular signaling and adhesion compared to lavandin EOs and EWMs, while lavandin EOs showed a slightly better effect against biofilm formation. Interestingly, the best antioxidant activity was determined for lavandin EWMs. A positive and moderate correlation was found between the reduction of <i>C. jejuni</i> intercellular signaling and adhesion, as well as between adhesion and biofilm formation. These findings mean novel bacterial targets are of interest for biofilm control with alternative natural agents throughout the whole food production chain.https://www.mdpi.com/2079-6382/11/7/854<i>Campylobacter jejuni</i>biofilmadhesionintercellular signalinglavandin formulations |
| spellingShingle | Dina Ramić Janja Ogrizek Franz Bucar Barbka Jeršek Miha Jeršek Sonja Smole Možina <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products Antibiotics <i>Campylobacter jejuni</i> biofilm adhesion intercellular signaling lavandin formulations |
| title | <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products |
| title_full | <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products |
| title_fullStr | <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products |
| title_full_unstemmed | <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products |
| title_short | <i>Campylobacter jejuni</i> Biofilm Control with Lavandin Essential Oils and By-Products |
| title_sort | i campylobacter jejuni i biofilm control with lavandin essential oils and by products |
| topic | <i>Campylobacter jejuni</i> biofilm adhesion intercellular signaling lavandin formulations |
| url | https://www.mdpi.com/2079-6382/11/7/854 |
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