Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species.

Quantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate quantification of gene expression to understand precise gene functions. A total of 25 candidate reference genes including traditional and new generation reference genes were selected and evaluated in a diverse set of c...

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Bibliographic Details
Main Authors: Dumbala Srinivas Reddy, Pooja Bhatnagar-Mathur, Palakolanu Sudhakar Reddy, Katamreddy Sri Cindhuri, Adusumalli Sivaji Ganesh, Kiran Kumar Sharma
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4749333?pdf=render