Cytoplasmic RNA Extraction from Fresh and Frozen Mammalian Tissues
The quality of collections of expressed sequence tags and full-length cDNAs is adversely affected by the presence of “junk” clones derived from unspliced or partially spliced RNAs present in conventional total RNA preparations. One can overcome this problem by using intact cytoplasmic RNA to create...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02332st01 |