Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus

<p>Abstract</p> <p>Background</p> <p>In this study, we developed a SYBR Green-based real-time PCR assay for the detection of sheep pox virus using a plasmid construct carrying one of the highly conserved genes encoding the virion envelope protein (P32) as a template.<...

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Bibliographic Details
Main Authors: Tian Hong, Wu Jingyan, Chen Yan, Zhang Keshan, Shang Youjun, Liu Xiangtao
Format: Article
Language:English
Published: BMC 2012-11-01
Series:Virology Journal
Subjects:
Online Access:http://www.virologyj.com/content/9/1/291